General Western Blot Reagents
R&D Systems Protein Normalization Module
The Protein Normalization Module from Simple Western is an accessory that enables automated normalization of protein levels across multiple samples for more accurate and reproducible western blot data. It works by probing a housekeeping target like actin or GAPDH in parallel with the protein of interest. The module consists of two chambers that allow simultaneous probing of the normalization protein and target protein. It is compatible with Wes, Simple Western's capillary electrophoresis and blotting system. The module delivers more accurate normalization than typical strip-based methods.
R&D Systems EZ Standard Pack 1, (8) Single Use Biotinylated Ladder and Single Use FL Standard
8 pk, EZ Standard Pack 1
R&D Systems Anti-Mouse Detection Module, 1ea
Use with a Wes or Peggy Sue/Sally Sue a Separation Module. Includes: Luminol-S, Peroxide, Antibody Diluent 2, Anti-Mouse Secondary Antibody, Streptavidin-HRP.
R&D Systems Fluorescent 5x Master Mix 1
Compatible with Jess, Abby, Wes, Peggy Sue and Sally Sue systems. Pack includes 8 packets with one each of the Single Use FL Standard (29 kDa system control protein) and Single Use DTT vials. Compatible with 12-230 Separation Modules.
LICORbio Revert™ 700 Total Protein Stain Kits for Western Blot Normalization (100 mL kit)
Make Western blot normalization more accurate and reliable with Revert 700 Total Protein Stain, a membrane-based (post-transfer) normalization strategy that stains all protein in your sample. Revert 700 Total Protein Stain works with your existing Western blot workflow and does not require special gels or equipment. The 100 mL Kit is enough for 20 mini blots and contains 1 x 100 mL Revert 700 Total Protein Stain, 1 x 200 mL Revert 700 Wash Solution, and 1 x 200 mL Revert Destaining Solution
LICORbio NewBlot™ IR Stripping Buffers for NIR Western Blots (100 mL)
NewBlot IR is a robust formulation optimized for near-infrared (NIR) Western blots that works with both nitrocellulose and PVDF membranes. This stripping buffer is a good place to start if you are new to stripping and reprobing NIR Western blots. NewBlot IR works well for removal of all IRDye secondary antibodies, and may be suitable for removal of other dye-conjugated secondary antibodies. NewBlot IR does not require hazardous shipping, unlike many other stripping buffers. Note: NewBlot IR Stripping Buffer is not recommended for loading amounts over 30 µg.
LICORbio Revert™ 700 Total Protein Stain Kits for Western Blot Normalization (250 mL kit)
Make Western blot normalization more accurate and reliable with Revert 700 Total Protein Stain, a membrane-based (post-transfer) normalization strategy that stains all protein in your sample. Revert 700 Total Protein Stain works with your existing Western blot workflow and does not require special gels or equipment. The 250 mL Kit is enough for 50 mini blots and contains 1 x 250 mL Revert 700 Total Protein Stain, 1 x 500 mL Revert 700 Wash Solution, and 1 x 500 mL Revert Destaining Solution
LICORbio CellTag™ 700 Stain for In-Cell Western™ Assays (2 x 10 nmol)
CellTag 700 Stain is a near-infrared fluorescent, non-specific cell stain that provides accurate normalization to cell number for In-Cell Western™ Assay applications. The stain accumulates in both the nucleus and cytoplasm of permeabilized cells, and provides linear fluorescent signal across a wide range of cell types and cell numbers. CellTag 700 Stain is detected in the 700 nm channel of Odyssey CLx, Classic, and Sa Imaging Systems. CellTag 700 Stain is applied to the cells during incubation with an IRDye 800CW secondary antibody, and enables accurate measurement of target protein levels with much higher throughput than Western blotting.
LICORbio Revert™ 520 Total Protein Stain for Western Blot Normalization (500 mL)
Make Western blot normalization more accurate and reliable with Revert 520 Total Protein Stain, a membrane-based (post-transfer) normalization strategy that stains all protein in your sample. Revert 520 Total Protein Stain works with your existing Western blot workflow and does not require special gels or equipment. Revert 520 Total Protein Stain is a robust and replicable solution for Western blot normalization. The stain can be detected in the 520 nm channel for quantification and normalization analyses. The protocol is quick and can be completed within 15 minutes. The 200 mL size is sufficient for 20 mini Western blots (5 mL/blot), and the 500 mL size is sufficient for 50 mini Western blots.
LICORbio NewBlot™ Nitro Stripping Buffer for Nitrocellulose Membranes (100 mL)
NewBlot Nitro is optimized for near-infrared (NIR) Western blots and is specially formulated for use with IRDye secondary antibodies, including IRDye 800CW, IRDye 680RD, and IRDye 680LT. NewBlot Nitro is intended for nitrocellulose membranes only, and is ideal for removal of secondary antibody.
Genscript Corporation WB-MASTER Protein Standard
WB-MASTER Protein Standard is designed for convenient protein identification in western blot. This standard consists of seven recombinant proteins with molecular weight of 20 kDa, 30 kDa, 40 kDa, 50 kDa, 60 kDa, 80 kDa and 120 kDa. Each of the proteins contains an IgG binding site that is able to bind to primary or secondary antibodies derived from a wide range of host species. The standard thus enables direct visualization of both the protein marker and users' samples on the same western blot membrane without any additional reagents.
LabScientific NONFAT DRY MILK standard for blotting. Molecular Biology Grade. Western blot. Biotin-Free. 2KG
Standard for all blotting: Western Blot Grade - Tested - Molecular Biology Grade Biotin-Free. 2KG
TOYOBO USA INC Can Get Signal | 250mL x 2 | Solution 1 & 2
Can Get Signal is a solution containing an accelerator for antigen-antibody reactions. This reagent improves sensitivity, specificity, and signal-to-noise ration (S/N) for Western blotting, dot blotting, enzyme-linked immunosorbent assay (ELISA), etc. Solutions 1 and 2 refer to the reactions of the primary and secondary antibodies, respectively.
